Application

Hyaluronidase has been used: to enzymatically remove extra-cellular matrix (ECM) to assess the lateral diffusion of the synaptic a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR)to study the effects of 4-methylumbelliferone (4MU)/ hyaluronidase (HLX)-mediated depletion of the CD44/hyaluronan complex on trastuzumab binding in JIMT1 breast cancer xenograftsas a component of Hank′s balanced salt solution (HBSS) medium to digest fetal intestine tissue

Biochem/physiol Actions

Hyaluronidase is an endoglycosidase that degrades hyaluronic acid (HA), a component of the extracellular matrix. It is used to enhance drug diffusion and reverse the effects of HA filler injections. Hyaluronidases dissolve the subcutaneous nodules and can correct excessive quantities of injected filler. It eliminates the cumulus-corona-oocyte complex formed during intracytoplasmic sperm injection. Hyaluronidase inhibits tissue damage after the extravasation of several substances. It is used for reducing edema treating vitreous hemorrhage.

General description

300 units/mg minimum, 50,000 units/vial. Suitable for biochemistry grade.Specifications:Activity: 300NF U/mg min Intended for laboratory and manufacturing use only. Not for drug, food, or household use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

1 pk in Glass ampoule

Physical form

Dialyzed, lyophilized powder, not less than300 units per mg (dry weight).

Unit Definition

One unit is based on the change in absorbency (turbidity) at 540 nm of an internal standard assayed concurrently with each lot. Internal standard replaces USP/NF reference no longer available.